ABSTRACT

The Present work was to develop a simple, fast, accurate, precise, reproducible, reverse phase high performance liquid chromatographic method for simultaneous estimation of metoprolol tartrate and telmisartan in pharmaceutical tablet dosage form marketed as telsar beta. Chromatographic separation was done using Agilent – Zorbax SB-CN RP C18 column having dimension of 4.6×150mm having particle size of 3.5μm, with mobile phase consisting of phosphate buffer pH 3 ±0.02 pH adjusted with ortho phosphoric acid and methanol (30:70 %v/v), flow rate was adjusted to 0.8 ml/min and detection wavelength at 226nm. The retention times of metoprolol tartrate and telmisartan was found to be 2.3 and 4.2mins. The Proposed method has been validated for accuracy, precision, linearity; range and robustness were within the acceptance limit according to ICH guidelines. Linearity for metoprolol tartrate and telmisartan was found in range of 25μg-125μg & 20μg-100μg and correlation coefficient was found to be 0.999 and 0.999, %RSD for intermediate precision was found to be 0.11 and for repeatability was 0.40 and 0.23, % mean recovery for metoprolol tartrate and telmisartan was found to be 99.8% to 100.5%. The method was found to be robust even by change in the mobile phase ±5% and in less flow condition. The developed method can be successfully employed for the routine analysis of metoprolol tartrate and telmisartan in API and Pharmaceutical dosage forms. Keywords: Metoprolol tartrate and Telmisartan, RP-HPLC, Method development, Method validation.

ABSTRACT

4-Carboxaldehyde-1-phenyl-3-methyl-2-pyrazoline-5-one condensed with Dinitro Phenyl hydrazine to form ligand (HL).These metal complexes are of type [ML2(H2O)2] [M= Mn(II), Co(II), Ni(II) and Cu(II)]. Elemental analysis, magnetic susceptibility, electrical conductance, electronic and infrared data suggest octahedral structure for the metal complexes. All the compounds were tested for their antimicrobial activity. The result indicates that the growth of the tested organisms was inhibited by most of the compounds.

Keywords: Dinitrophenlhydrazone, Antimicrobial activity, Complexes.

ABSTRACT

Plants are most attractive biological resources because of their ability to produce a huge variety of chemical compounds, and the familiarity of production in even the most rural settings. Alkaloids, terpenoids, steroids, polyketides, phenylpropanoids and flavonoids are among the economically important compounds derived from plants. By comparison, bacteria and mammals together produce around 3000 economic natural products. Agronomic traits such as herbicide tolerance, insecticides, nematodes resistance, disease-tolerance, and delayed ripening, were initial focus of crop improvement during these days through genetic recombinant DNA technology. Among all the crop quality damaging parasites, nematode infection occupies a most significant position. In this paper, we review the status of neutraceuticals under nematode infection.

Keywords: Rec-DNA technology, Meloidogyne incognita, Improvement, Hormones and Vitamins.

ABSTRACT

The mixed ligand complexes of Co(II), Ni(II), Cu(II) and Zn(II) with Schiff bases N-(2-hydroxy-1-naphthylidene)-4-nitroaniline (L1H) and N-(2-hydroxybenzylidene)-2,3-dimethylaniline (L2H) have been synthesized and characterized. The resulting complexes were characterized by elemental analysis, thermogravimetric analysis, magnetic moment measurements, conductivity measurements, 1H NMR, IR, UV-visible and ESR spectral studies. The Schiff bases acts as bidentate monobasic ligands, coordinating through deprotonated phenolic oxygen and azomethine nitrogen atoms. The complexes are non-electrolytic in DMSO. The presence of the two coordinated water molecules in these complexes was indicated by IR spectra and thermogravimetric analysis of the complexes. From the analytical and spectral data the stoichiometry of these complexes have been found to be [M(L1)(L2)(H2O)2] {where M = Co(II) , Ni(II), Cu(II) and Zn(II)}. It is found that Co(II), Ni(II), Cu(II) and Zn(II) complexes exhibited octahedral geometry. The antimicrobial activities of ligands and their mixed ligand complexes were screened by Disc Diffusion method. It was found that the metal complexes have higher antimicrobial activity than the free ligand.

Keywords: Schiff bases, Mixed ligand, Metal complexes, Antimicrobial activity.

ABSTRACT In pharmaceutical analysis both for qualitative and quantitative analysis reagents are important, various reagents are routinely used in qualitative and quantitative analysis of pharmaceuticals. In this section we focussed on principles and procedures of some important reagents used in pharmaceutical analysis. 1, 2-naphthoquinone-4-sulfonate is a chemical regent used to measure levels of amines and amino acids like ethylenimine limit test, colorimetric determination of cefadroxil and ceftriazone in pharmaceutical dosage forms and spectrophotometric determination of pregabalin using 1, 2-napthaquinone-4-sulfonic acid sodium. 2,3,5-triphenyltetrazolium is redox indicator used in Seed analysis and Quantitative determination of effects of Mycoplasma pneumonia cells and Membranes.(study of the infective process in primary atypical pneumonia) and so many regent used in pharmaceutical analysis. 3-methylbenzthiazolinone-2(3H)-hydrazone originally introduced as a reagent for aldehyde Analysis of Acyclovir, Analysis of Ceftazidime, Analysis of Ganciclovir and Analysis of Cefadroxil.

Keywords: 1, 2-naphthoquinone-4-sulfonate, 2, 3, 5-triphenyltetrazolium, 3-methylbenzthiazolinone-2(3H)-hydrazone, Cefadroxil, Mycoplasma pneumonia and Ceftazidime.

ABSTRACT

The aim of this study was strain improvement of Bacillus pumilus for Carboxy Methyl Cellulase production by physical mutation (UV irradiation) and Chemical mutation (Ethyl Methyl Sulphate).The wild Bacillus pumilus strain isolated from earthworm gut produced 0.665 ± 0.004 IU/ml of CMCase. After UV irradiation Bacillus pumilus(UVR10) exhibited high efficiency of CMCase production (1.330 ± 0.075 IU/ml).The UV mutant Bacillus pumilus was further subjected to chemical mutation in EMS (200 μg/ml)at different time intervals. The least survival was recorded at an exposure period of 120 minutes. In this regard, Bacillus pumilus CR24 exhibited high efficiency on the basis of the CMCase production (2.610 ± 0.093 IU/ml).The mutant strain of Bacillus pumilus effectively produced higher amount of CMCase than wild Bacillus pumilus strain.

Keywords: UV irradiation, EMS, Bacillus pumilus, CMCase, Cellulose.

ABSTRACT

Garden cress (Lepidium sativum Linn) is a polymorphous species, considered to be originated primarily in Europe and Western Asia. The seeds are important as medicinal crops and are cultivated in Arabia, Albania, Tibet, Syria, Greece and India. Garden cress is fast growing edible plant. All the parts of garden cress, i.e., seeds, leaves and roots are of enormous economic importance, however, the crop is mainly cultivated for seeds. The seeds are good source of protein, fat, calcium, ir on and phosphorous. The properties of the seeds are described as rubifacient, tonic, laxative, aphrodisiac, diuretic, mucopurulent and galactogogue. The seeds are also used in the treatment of diarrhoea, diabetes, high blood pressure, respiratory disturbances, rapid fracture healing, hypercholesterolemia and anaemia. The present comprehensive review work is specially focused on nutritional evaluation and medicinal properties of garden cress seeds for better application in various therapeutic research.

Keywords: Lepidium sativum, Galactogogue, Antianaemic, Antihypertensive.

ABSTRACT

Medicinal plants have been a major source of treatment for human diseases since time immemorial. The physicochemical evaluation of the plant material is an important parameter in detecting adulteration or improper handling of drugs. The present study was planned to compare the phyto-physicochemical analysis of Enicostemma littorale Blume (Whole plant) with Withania somnifera (L) Dunal (root). Both plants are medicinally important and used as anti-arthritic, anti-inflammatory, antioxidant and hepatoprotective. These plant materials were phyto, physico-chemically standardized according to the methods recommended by the World Health Organization and standard laboratory procedures. The highest percentage of hot water and ethanol extractable matter; total ash and acid insoluble ash (37.21±1.27% & 24.92±0.64%, 8.16± 0.1% & 1.89±0.1%) were found in E. littorale when compared with W. somnifera (30.82±1.09% & 10.0±0.21%, 5.76± 0.1% & 0.63±0.04%). Percentage of loss on drying was found in W. somnifera (9.12±0.10%) and it was slightly lower than E. littorale (10.25±0.33%). Preliminary phytochemical screening of these both plant materials revealed that presence of alkaloids, saponins, flavonoids, steroids, tannins, proteins, reducing sugar, fat and fixed oils and coumarins. This comparative information provides immense potential for studying their activities for arthritis and other disease conditions, both in pre-clinical and clinical stages, which lead to the preparation of useful pharmaceutical products. The present study has authenticated the usefulness of the identified plants for medicinal purposes. These species could also be seen as potential source of useful drugs due to their rich contents of phytochemical.

Keywords: Comparative study, Physicochemical, Phytochemical, Enicostemma littorale, Withania somnifera.

ABSTRACT

Ficus bengalensis, a genus of family Moraceae is a tropical, deciduous, evergreen tree with more than 800 species and about 40 genera of the mulberry family. F.bengalensis is known as common name Bargad and cultivated as a Garden tree or Spiritual tree. The leaves of F. bengalensis is used as ulcer protective, leprosy and fever, inflammations(Ayurvedic).The milky juice is aphrodisiac, tonic, vulnerary, maturant also useful in piles, diseases of the nose, gonorrhea. In Yunani the aerial root is styptic, syphilis, biliousness, dysentery, inflammation of liver. A lot of pharmacological work has been scientifically carried out on various part of F.bengalensis but some other traditionally important therapeutically uses are also remaining to proof till now scientifically. As analgesic, antipyretic, anti-ulcerogenic, inflammatory bowel, antimicrobial, antidiabetic etc. The various chemical constituents present in F.bengalensis are Bengalenosides, flavanoids and leucocyanidin glycoside.

Keywords: Ficus bengalensis, Moraceae, Antimicrobial, Diabetes, Antioxidant.

ABSTRACT

An investigation was undertaken to study the Physical and Chemical properties of coal in Korba district. Due to the Presence of lot of Coal mines, number of coal based thermal power stations are established in Korba district. So study has been carried out for assessment of coal quality, whether it is suitable for thermal power stations, by collectying sample from Gevra Coal mines. This paper presents Grade of the coal available in Korba district. Three different Coal samples were collected from different areas of Gevra Coal mines and analyzed for Proximate, Ultimate and Calorific value as per Standard methods. The useful heat values (UHV) of three coal samples are 2482K.Cal/Kg, 2917K.Cal/Kg, and 2786K.Cal/Kg. From overall analysis and according to UHV of coal samples we can conclude that the grade of Gevra Coal is “F” and is very much useful for Coal based thermal power stations.

Keywords: Coal mines, Chemical properties, Thermal power, Calorific value.