| 1. | METHOD DEVELOPMENT AND VALIDATION OF
SIMULTANEOUS ESTIMATION OF DROSPIRENONE AND
ESTETROL BY USING UV-SPECTROSCOPY |
| Garamsandh Gandhi V*, Manivannan R, Blessalya M, Boopathi S, Deepak Kumar A,
Harish K, Yaswanth PS |
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Ultraviolet and visible (UV-Vis) absorption spectroscopy is the measurement of the attenuation of a beamof light
after it passes through a sample or after reflection from a sample surface. Light energy is converted by detectors into
electrical impulses that are read out by readout devices. The transmitted radiation strikes the detector, determining the
amount of radiation absorbed by the sample. The absorption spectrophotometer's apparatus uses the following types of
detectors. Aim of the present study is to develop an accurate, precise, sensitive, selectivity, analyticaltechnique to determine
the Drospirenone and Estetrol in pharmaceutical dosage forms. Drospirenone is a progestin used in oral contraceptive pills for
the prevention of pregnancyand other conditions. Percentage of RSD for intra-day and inter-day precision studies for both
drugs were well within the acceptable range (< 2 %) indicating that the method have excellent repeatability and
reproducibility
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| 2. | NALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE
SIMULTANEOUS ESTIMATION OF DECITABINE AND CEDAZURIDINE IN
BULK AND PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD |
| Yamini S*1, Rajitha S |
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High performance liquid chromatography is at present one of the most sophisticated tool of the analysis. The estimation of
Decitabine and Cedazuridine was done by RP-HPLC. The Phosphate buffer was pH 4.5 and the mobile phase was optimized
with consists of Methanol: Phosphate buffer mixed in the ratio of PH 4.5(20:80 v/v). Kromosil C18 Column (250mm x
4.6mm)5µg or equivalent chemically bonded to porous silica particles was used as stationary phase. The detection was carried
out using UV detector at 254 nm. The solutions were chromatographed at a constant flow rate of 1 ml min-1. The linearity
range of Decitabine and Cedazuridine were found to be from 100-500 mg/ml of Decitabine and 1-5mg/ml of Cedazuridine.
Linear regression coefficient was not more than 0.999. The values of % RSD are less than 2% indicating accuracy and
precision of the method. Decitabine %RSD 0.2 and Cedazuridine %RSD 0.6. Intermediate precision for Decitabine %RSD 0.2
and Cedazuridine %RSD 0.1 The percentage recovery varies from 98-102% of Decitabine and Cedazuridine. LOD and LOQ
were found to be within limit. The results obtained on the validation parameters met ICH and USP requirements .it inferred the
method found to be simple, accurate, precise and linear. The method was found to be having suitable application in routine
laboratory analysis with high degree of accuracy and precision
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