Lupeol is a triterpenoid pentacyclic compound that is known for its anti-inflammatory and anti-cancer properties. Its activities are based on its anti-oxidant potential. Lupeol has been found to have a contraceptive effect due to its inhibiting effect on the calcium channel of sperm (CatSper). Lupeol has also been shown to exert anti-angiogenic and anti-cancer effects via the downregulation of TNF-alpha and VEGFR-2. Famous anti-inflammatory ethno-medicinal plant Camellia japonica contains anti-inflammatory component lupeol in its leaf. In order to determine the drug in biological fluid or in pharmaceutical preparations, there are no. of methods available, that is HPTLC, HPLC, and spectrophotometery. It can be concluded that the proposed method is simple, rapid, accurate, precise, economic and reproducible for UV spectrophotometric estimation of lupeol from pharmaceutical formulation. This method can be successfully applied for routine estimation of lupeol in bulk and pharmaceutical dosage form.