Abstract

A new, simple and sensitive spectrophotometric method in visible region has been developed for the determination of Lamivudine in bulk and in pharmaceutical formulations. Lamivudine   exhibits absorption maxima at 425 nm. Developed method obeyed the Beer’s law in the concentration range of 5 - 20 μg/mL. The method is accurate, precise and economical. The proposed method has been applied successfully for the analysis of the drug in pure and in its tablet dosage forms. In this method, there is no interference from any common pharmaceutical additives and diluents. The % recovery is greater than 99 to101%. %, this shows that the method was free from the interference of excepients. The results of the tablet analysis were validated with respect to accuracy (recovery), linearity, limit of detection and limit of quantization were found to be satisfactory.

Keywords: UV Spectrophotometry, Lamivudine, Colorimetric Estimation, Lamivudine Tablet.

ABSTRACT

Phoenix sylvestris Roxb. a member of the Arecaceae family, is used in ayurveda because of its treatment in tooth ache, urinary disorders, digestive disorders and ulcer. The purpose of the present study is to investigate the acute oral toxicity and anti-ulcer profile of the Ethanol Extract of Phoenix sylvestris Roxb. (EPS) extract in albino rats. Study on acute toxicity of extract found to be safe at the doses 2000mg/kg body weight orally as per OECD guidelines No.423. EPS at the doses of 200 and 400 mg/kg body weight orally was administered to evaluate anti-ulcer activity by using Ethanol, indomethacin, pyloric ligation (PL), and cold-restraint stress induced gastric ulcer models in Albino rats. Ethanol Extract of Phoenix sylvestris Roxb. dose dependent inhibition in Ethanol induced gastric lesions, causing 65.89% protection at 400 mg/kg, and 47.87% protection at 200 mg/kg, EPS dose dependent inhibition in indomethacin induced gastric lesions, causing 62.79% protection at 400 mg/kg and 56.88% protection at 200 mg/kg, EPS dose dependent inhibition in pylorus ligation induced gastric ulcer (PL) causing 70.07% protection at 400 mg/kg and 45.41% protection at 200 mg/kg and it also dose dependent inhibition in Cold-restraint stress induced gastric lesions, causing 72.02% protection at 400 mg/kg, and 54.96% protection at 200 mg/kg. All the results are found to be statistically significant (p≤0.05). Hence we suggest that Ethanol Extract of the root of Phoenix sylvestris Roxb. possess anti-ulcer properties that may be due to cytoprotective mechanism. These results support the ethnomedical uses of the plant in the treatment of gastric ulcer.

Keywords: Phoenix sylvestris Roxb, Anti-Ulcerogenic Activities.

ABSTRACT

This article presents a review of basic chromatographic techniques applied to the determination of various pharmaceuticals is discussed. It describes about various Chromatographic techniques and is usage. Also it briefly describes about the instruments, methods used in it. Chromatographic separations can be carried out using a variety of supports, including immobilized silica on glass plates (thin layer chromatography), volatile gases (gas chromatography), paper (paper chromatography), and liquids which may incorporate hydrophilic, insoluble molecules (liquid chromatography).

Keywords: Chromatography, HPLC, TLC, GC, Method development, Validation.

ABSTRACT

This review in analytical method development by HPLC is aimed at analysts who have been performing HPLC troubleshooting and method development in Pharmaceuticals. We are trying to present an in depth study of method development primarily by HPLC method. The technique that we are discussing here is RP-HPLC. This technique offers flexibility in the use of a variety of columns, detectors, pumps etc. as per the drug requirement.

Keywords: HPLC, Method development, Validation, Chromatography.

ABSTRACT

A series of 1-ethyl/benzyl-6-fluoro 7-[4- (-N- 4- substituted phenyl) benzene sulfonamide)1-Piperazinyl] -1,4, dihydro -4- oxo-quinoline carboxylic acid (C1-C8) were synthesized and characterized by IR, MASS, NMR spectral and elemental analyses. All the compounds screened for their in vitro antibacterial and antifungal activities by paper disc diffusion method. The Minimum inhibitory concentration (MIC) of the compounds was determined by broth dilution technique. The in vivo antibacterial activity of the compounds against E.coli and Staphylococcus aureus was evaluated by mouse protection method. Compounds C1, C2, C5 and C7 exhibited very good antimicrobial activity. Compounds C1, C3 and C5 showed good bactericidal and fungicidal activities.

Keywords: Fluoroquinolone, Anti – bacterial, Topoisomerases, Ciprofloxacin.

ABSTRACT

A simple, specific, accurate and precise RP HPLC method has been developed for the simultaneous determination of Losartan Potassium (LOS) and Hydrochlorothiazide (HCTZ) from combined dosage form by reverse phase C18 column (Prontosil; CN (250mm x 4.6mm) 5μ). The sample was analysed using Triethylamine: Acetonitrile: Methanol in the ratio of 30:30:40(pH adjusted to 7.0 with phosphric acid) as a mobile phase at a flow rate of 1.0ml/min and detection at 270nm. The retention time for Losartan potassium (LOS) and Hydrochlorothiazide (HCTZ) was found to be 11.869 min and 7.893 min respectively. The stability assay was performed for this combination and was validated for accuracy, precision, linearity, specificity and sensitivity in accordance with ICH guidelines. Validation revealed the method is specific, rapid, accurate, precise, reliable, and reproducible. Calibration plots were linear over the 70%-130% concentration ranges for both the drugs of LOS and HCTZ respectively, and recoveries from combined dosage form were between 98 and 102%.

Keywords: Losartan Potassium, Hydrochlorothiazide, RP-HPLC, Estimation.

ABSTRACT

The present review describes about method develop and validate a bioanalytical method in plasma. A HPLC method with UV-visible detection by employing C18 column can be used do estimate the drugs present in plasma with 10 mM ammonium acetate buffer and methanol as mobile phase. The developed method has to validate as per the USFDA guidelines for bioanalytical method validation.

Keywords: Bioanalytical method, Recent Analytical Techniques, HPLC, Plasma.