ABSTRACT

The aim of the present study was to isolate and determine the amino acids from different extracts of Cicer arietinum leaves belongs to the family Fabaceae by LC-MS technique. It is an annual herb that is spread into Southern Europe, India, Egypt and Southern America. It is extensively cultivated in India mainly in Rajasthan, Hyderabad, Patiala, East Punjab, Haryana and Madhya Pradesh. In India it is very often used as a crash diet and it is one of the most widely made recipes in India kitchen due to its good taste and nutritive values. Traditionally it is used as antibacterial, antifungal, antipyretic, antidiarrhoeal etc. The presence of amino acids has been for the first time evaluated by a modern technique like LC-MS technique. The method presented above provides an important tool for the qualitative evaluation of important amino acids from Cicer arietinum.

Keywords: Cicer arietinum, Amino acids, LC-MS technique.

ABSTRACT

A sensitive, accurate and economical UV spectrophotometric method with multivariate calibration technique for the determination of almotriptan malate in bulk drug and pharmaceutical formulation has been described. This technique is based on the use of the linear regression equations by using relationship between concentration and absorbance at six different wavelengths. The results were treated statistically and were found highly accurate, precise and reproducible. The method is accurate, precise and linear within the range 2-10μg/ml. There was no interference from the excipients. This statistical approach gives optimum results for the eliminating fluctuations coming from instrumental or experimental conditions.

Keywords: UV Spectrophotometry; Multivariate Calibration; Pharmaceutical Formulation.

ABSTRACT

Two simple, accurate and precise UV methods were developed for the estimation of Gemifloxacin (GEM) and Ambroxol hydrochloride (AMB) from its tablet dosage form. Both the drugs are used in treatment of chronic bronchitis and mild to moderate pneumonia. Method I is Simultaneous equation method, wavelengths selected for Quantitation are 271.0 nm and 245.5 nm for c respectively which are the λ_max of both the drugs. Method II is Q –Analysis method, wavelengths selected were 271nm (λmax of GEM) and 244.0 nm (Isobestic point) for the analysis. In both the methods linearity for detector response was observed in the concentration range of 10-60 µg/ml for GEM and2-12 µg/ml for AMB respectively. The results of tablet analysis for method I is found to be 99.97% + 0.041 S.D for GEM and 99.93% + 0.21 S.D for AMB and results obtained for Method II is 99.94% + 0.080 S.D for GEM and 99.90% + 0.15 S.D for AMB. The proposed methods were successfully applied for the simultaneous determination of both the drugs in commercial tablet preparation. The results of the analysis have been validated statistically.

Keywords: Gemifloxacin mesylate, Ambroxol HCl, UV-Spectrophotometry, Simultaneous equation method, Q-Analysis method.

ABSTRACT

The previous studies showed that UV method has not reported for Asenapine maleate in their pharmaceutical preparation. Therefore, the present study we developed UV method for the determination of Asenapine maleate in tablets. Asenapine maleate shows good solubility in organic solvents. Chemically asenapine maleate is (3aRS,12bRS)-5-Chloro-2,3,3a,12b-tetrahydro-2-methyl-1H dibenz[2,3:6,7]oxepino[4,5-c]pyrrole (2Z)-2-butenedioate(1:1). The present work objective was to develop a validated UV Spectroscopic method for asenapine maleate drug in bulk form and in tablet dosage form. The suitable solvent selected for performing estimation of asenapine maleate by UV spectroscopic method development and validation and fixed the λmax for the drug asenapine maleate. the present study successfully estimated the asenapine maleate from the formulation and performed validation studies of the drug asenapine maleate.

Keywords: Asenapine maleate, UV spectroscopic method, validation studies.

ABSTRACT

Cocrystal is a crystalline structure consisting of two or more components that form a unique structure having specific properties. The physical and chemical property improvements through pharmaceutical co-crystals draw closer the fields of crystal engineering and pharmaceutical sciences. A pharmaceutical co-crystal is a single crystalline solid that incorporates two neutral molecules, one being an active pharmaceutical ingredient (API) and the other a co-crystal former. This technology is used to identify and develop new proprietary forms of widely prescribed drugs and offer a chance to increase the number of forms of an API. This review focuses on properties of cocrystals, their methods of synthesis and applications in the field of pharmacy.

Keywords: Cocrystal, crystal engineering, drug, solid.

ABSTRACT

Literature survey reveals that few analytical methods were reported for the estimation of diflunisal by LC method. Hence the present study aims in developing simple, rapid, precise and validated methods for diflunisal in formulations. Organic solvents causes environmental hazards so, to have green synthesis selection of hydrotropic solubilizing agent was preferred. This current investigation is intended to develop a method to determine the assay by U.V. Spectrophotometry, for both bulk of Diflunisal. This method involves direct analysis without any extraction steps, thus it is performed faster, simple and easier. And this method is shown accurate and précised results. By these results this method found to be rapid, simple, accurate, economic method for analysis and quality determination.

Keywords: Diflunisal, UV spectroscopic method, validation studies.

ABSTRACT

Docking is frequently used to predict the binding orientation of small molecule drug candidates to their protein targets in order to in turn predict the affinity and activity of the small molecule. Hence docking plays an important role in the rational design of drugs. Given the biological and pharmaceutical significance of molecular docking, considerable efforts have been directed towards improving the methods used to predict docking. This present review focused on importance of docking in molecular modeling, types of docking and application of docking in varies fields.

Keywords: Docking, Macromolecular docking, Applications.