ABSTRACT

A novel isocratic reverse phase liquid chromatography method for determination of Linagliptin was developed and validated after optimization of various chromatographic conditions. A Khromosil  C18, 5μm column having 150×4.6 mm i.d., with mobile phase containing 0.02 M potassium dihydrogen phosphate : acetonitrile (70:30, v/v, pH  5.0 adjusted with 1%  OPA solution) was used. The flow rate was 1.2 mL min^-1and effluents were monitored at 226 nm. The retention time of Linagliptin was 4.2min. The linearity for Linagliptin was in the range of 0-75μg mL^-1 with coefficient of correlation 0.999. The proposed method was validated with respect to linearity, accuracy, precision and robustness.

Keywords: Linagliptin, RP- HPLC, Tradgenta Tablets, Validation.

ABSTRACT

A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for voriconazole in parental dosage form. The method was carried out on an Intersil ODS-C₁₈ (150X4.6X5µ) column with a mobile phase consisting of mixed phosphate buffer, ACN and Methanol (65:30:5) and flow rate of 2.0 mL min-1. Detection was carried out at 257 nm. The retention time for VCZ was found to be 6.413 min. The VCZ % recovery was within the range between 99.55-99.63%. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per ICH guidelines. The developed method was validated for precision, accuracy, sensitivity and robustness. The developed method can be used for routine analysis of titled drug in formulation.

Keywords: Voriconazole; RP-HPLC, Intersil ODS, Validation.

ABSTRACT

Quinazoline is the main fused heterocyclic ring system reported for their biological activities, compounds with varieties of pharmacophores, which bring together knowledge of a target with understanding of the molecule types that might interact with the target family. The molecular manipulation of promising lead compound is still a major line of approach for the discovery of new drugs. Combination of two or more moieties into one is a common procedure of manipulation and this can probably result in the increase of biological activity and removal of untoward side effects.

Keywords: Quinazoline, Pharmacophores, Discovery.

ABSTRACT

Cilostazol is an anti – thrombotic drug which is used in the treatment of peripheral vascular disease. A sensitive and accurate UV- Spectrophotometric method with Multi – Variate calibration technique for the determination of Cilostazol in Bulk drug and Pharmaceutical Formulation has been described. This technique is based on the use of linear regression by using relation between concentration and absorbance at five different wavelengths. The wavelength of maximum absorbance of Cilostazol was found to be 256 nm and the five wavelengths selected were 252 nm, 254 nm, 256nm, 258 nm, 260 nm. The results were treated statistically and were found to be accurate, precise, and reproducible. The method is accurate, (% recovery 99.3% - 101.8%), precise (% RSD NMT 2.5), correlation coefficient was 0.9994 and linear within the range of 2 – 20 μg/mL, LOD was 0.483 μg/mL and LOD was 1.463 μg/mL. There was no interference from the excipients.  This statistical approach gives optimum results for eliminating errors due to instrumental fluctuations.

Keywords: Cilostazol, Multi- Variate Calibration, UV-  spectrophotometry.

ABSTRACT

Nanoscience is one of the most important research and development frontiers in modern science. The word ‘nano’ means one billionth, and the nanometre (nm, 10^–9 m) defines the length scale that is used to measure systems being studied in nanoscience. In the most simplistic sense, nanoscience is the science of small particles of materials. Such small particles are of interest from a fundamental viewpoint because all properties of a material, such as its melting point, and its electronic and optical properties, change when the size of the particles that makes up the material become nanoscopic.

Keywords: Nanoscience, Nano, SLNs, Cosmeceuticals.

ABSTRACT

SSRI discontinuation syndrome, in light of the multitude of prescribed agents, has been disputed. SSRI discontinuation syndrome result from a global downregulation of serotonin receptors in response to increased levels of serotonin in the synaptic cleft, but the specific mechanism through which this creates symptoms is not understood. This review discussed on clinically significant indications of difficulties with the discontinuing of SSRIs, history, mechanism of action, prevention and treatment.

Keywords: SSRI discontinuation syndrome, Prevention, Treatment.