ABSTRACT Psoriasis is an inflammatory dermatosis that is characterized with hyper proliferation of keratinocytes and inflammatory infiltration in the epidermis and dermis. Evidences is now accumulating that there is an association between Calcium, Phosphorus, Magnesium and Psoriasis but the detailed mechanisms are not yet known. Low serum Calcium level and high serum phosphorus level has been suggested in the pathogenesis of his phenomenon. In this study we estimate Calcium, Phosphorous and Magnesium values in Psoriatic patients and compare with those of age and sex-matched non Psoriatic controls. The study involved a control group of apparently healthy non-psoriatic as controls (N = 40) matched for age with a test group of psoriatic patients (N = 60). The age range of both groups was 08-65 years. Serum total calcium (Ca++), Phosphorus (PO4), , and Magnesium (Mg++), concentrations were measured according to the standards. Appropriate statistical tests were used to assess significant difference in the means of the studied concentrations between patients and the control group. The psoriatic patients showed significantly lower T.Ca++ (M±SD=8.29±0.72mg/dl) , Significantly higher T.PO4 (M±SD=4.5±0.40mg/dl) level compared to non- psoriatic (M±SD=9.96±1.04 mg/dl, M±SD=3.6±0.58 mg/dl respectively P<0.05). In contrast there is no significant difference in Psoriatic Mg++ level (M±SD=1.39±0.19mg/dl), compared to non- psoriatic (M±SD=1.38±0.17mg/dl).P>0.05. In this study we concluded that the decrease in above parameters are the probable causative agent for the pathogenesis of Psoriasis and may be useful to do early screening and mineral treatment to prevent its complications. Keywords: Psoriasis, Calcium, Phosphorus, Magnesium.

ABSTRACT A new series of unsymmetrical macrocyclic binuclear Copper (II) complexes have been synthesized and characterized by elemental and spectral techniques. A cyclic voltammetric investigation of these binuclear Cu (II) complexes evidenced that two successive quasi-reversible one electron transfer reduction waves (E1pc = -0.54 to -0.67 V, E2pc = -0.80 to _0.85 V) are obtained. In the positive potential region (+0.60 to +1.2 V) two quasi-reversible oxidation couples are observed for all the complexes. The first one electron oxidation is observed around (E1pc) +0.59 to +0.79 V and the second around (E2pc ) +0.81 to + 1.17 V. The ESR spectra of all the binuclear copper complexes showed a single broad-band resonance at ca. g = 1.98 to 2.11 with the half field signal at 1500G (M = ±2), which suggest magnetic interactions between two Copper ions through the phenolate bridge. DNA binding experiments show that the macrocyclic CuII complexes display better DNA interactions with increasing order of the chain length. Keywords: Copper (II) complexes, Elemental and Spectral techniques.

ABSTRACT A Simple, accurate and rapid RP-HPLC method has been developed for the estimation of Gliclazide (GLC) in bulk and pharmaceutical dosage forms using an XDB C 18, 150 x 4.6 mm i.d, 5 μm particle size in isocratic mode; with mobile phase comprising of buffer (0.02M potassium dihydrogen phosphate) and acetonitrile in the ratio 55:45 (v/v). The flow rate was 1 ml/min and detection was carried out by photodiode array detector at 228 nm. The retention time for GLIC was found to be 5.078 min. The proposed method has permitted the quantification of GLIC over linearity in the range of 25 – 150 μg/ml and its percentage recovery was found to be 99.21 – 100.419 %. The % RSD of intraday and inter day precision were found 0.4433% and 0.1%. This RP – HPLC method is simple, single and reproducible, with high resolution and has been successfully applied for the determination of GLC. Keywords: Gliclazide, RP-HPLC, Validation and method validation.

ABSTRACT A Simple, precise and accurate method was developed for the estimation of Tinidazole, Ciprofloxacin and related impurities (ethylenediamine) analysis.For RP-HPLC method Mobile phase – A is pH 4.5 KH2PO4 buffer and mobile phase-B is PH 4.5 Potassium dihydrogen phosphate buffer and Acetonitrile in the ratio of 50:50%v/v was selected as a mobile phase gave retention times 21.93, 11.02 and 18.47 respectively for Ciprofloxacin, Tinidazole and Ethylenediamine. The column used was X-Terra C18 column, 150 × 4.5mm i.d, 5μm with flow rate 1ml/min using UV detection at 278 nm &317nm. The correlation coefficient of Ciprofloxacin, Tinidazole and Ethylenediamine was found to be 0.999 .The limit of quantification for Ciprofloxacin, Tinidazole and Ethylenediamine was found to be 0.1050μg/ml, 0.1530μg/ml and 0.1190 μg/ml respectively. The accuracy was found to be within the limits. The precision was with in the acceptance criteria not more than 15.0% for each individual impurity. Hence it is conclude the developed RP-HPLC method can be effectively used for estimation of Ciprofloxacin, Tinidazole and and their related impurities from pharmaceutical dosage forms.

Keywords: RP-HPLC, Tinidazole, Ciprofloxacin, Ethylenediamine.

ABSTRACT The present study was aimed to evaluate the cardio protective activity of 3-(benzoxazol-2-ylimino)-5-fluoroindolin-2-one against the Doxorubicin (15mg/kg, i.p, single dose) induced cardiotoxicity in rats. Four groups of female wistar albino rats were used. Group 1 was used as control (0.5% CMC, oral); the other groups were treated with Doxorubicin (single i.p. dosage of 15mg/kg) or Doxorubicin plus test compound (50mg/kg/day & 100mg/kg/day, p.o) respectively. Animals were treated with test Compound or CMC for 7 days. On 6th day, a single dose of DOX was administered to rats. Blood was collected on 7th day. Evaluation of cardioprotective activity was done by estimating biochemical parameters like plasma Aspartate aminotransferase (AST), Creatinine kinase (CK-MB), Lactic acid dehydrogenase (LDH) and Triglyceride (TG) levels. Pre-treatment with test compound significantly reduced the elevated levels of cardiotoxic biomarkers in plasma. Keywords: Cardiotoxicity; Cardioprotective; 3-(Benzoxazol-2-ylimino)-5-fluoroindolin-2-one; Doxorubicin; LDH; CK-MB; AST; TG.

ABSTRACT A new validated potentiometric method for determination of thiamine hydrochloride (TH) was developed. The developed method was based on the construction of two types of sensors; coated wire sensor (I) and coated graphite sensor (II). The fabricated sensors were performed using TH-tetraphenylborate (TH-TPB) as electroactive material. All performance characteristics of the fabricated sensors were investigated. The fabricated sensors exhibited Nernstain response (31.7±0.5 and 31.9±0.3 mV decade-1) over concentration ranges of 1.0×10-6-1.0×10-1 mol L-1 and 1.0×10-5-1.0×10-1 mol L-1 with limits of detection of 2.7×10-7 and 2.5×10-6 mol L-1 for sensor I and II, respectively. The recorded pH range was 3.5-7.5 for both sensors. The interference of some cations, anions, sugars, amino acids and some pharmaceutical co-formulations were tested, also the effect of some pharmacological action related drugs were studied and no interferences were recorded. The fabricated sensors displayed good isothermal coefficients 0.00165 and -0.00061 V â—‹C-1. The proposed method showed good results and these results were statistically treated and compared with those obtained from other reported methods. The analytical applications were performed using the fabricated sensors in the determination of TH in dosage forms and biological fluids. Keywords: Potentiometric method, Thiamine hydrochloride, Coated wire sensor, Coated graphite sensor, Pharmaceutical formulations, Biological fluids.

ABSTRACT A novel series of 2-aryl-3-(5-phenyl[1,2,4]triazolo[4,3-c]quinazolin-3-yl)-1,3-thiazolidin-4-ones 4a-k with varied electronic environment due to aryl moiety present on 2nd position of thiazolidinone has been designed, synthesized by one-pot three-component synthesis and characterized by their spectral data. In-silico molecular docking studies are carried out using low energy conformer of compounds 4a-k in the active domain of Hepatitis C virus NS5B RNA polymerase (protein ID: 3FRZ). Their aptness in regulating the functions were studied, from their interaction energies, calculated using the scoring functions, depicted that the ligands 4h (–7.813) and 4j (–7.885), bearing 2-chlorophenyl and 2-furyl substituents respectively, showed most fitting interactions than the standard filibuvir (–7.764), establishing as good inhibitors of HCV (Hepatitis C virus) and may evolve as potent antiviral agents. Keywords: Thiazolidin-4-one derivatives, Synthesis, Molecular docking, Hepatitis C virus NS5B RNA polymerase inhibitors.

ABSTRACT The present investigations discuss the correlation between concentrations of active acetyl salicylate moiety in the synthesized tablet and solvation rates in different pH-media. Furthermore AFM-microscopy was to visualize a real 3D-imaging of sample’s surface topography .High resolution AFM-investigations indicated that crystalline aspirin has regular arrays of atomic arrangement with no violation in the bulk of aspirin.TM deflection AFM- gave us good approximation to the diffusion of grain throughout the surface topology of investigated aspirin. 3D-visualized imaging is introducing precise determination of exposure surface area interacting with dissolving agent responsible for increasing or decreasing calculated solvation rates. Keywords: AFM-microscopy , pH-effect ,Grain , Nano-structural Features ,Deflection centers.

ABSTRACT

A new, simple and highly sensitive coated wire sensors for direct determination of cyanocobalamin (CYN) were developed. The developed sensors were based on the fabrication of three different kinds of sensors, using cyanocobalamin-phosphomolybdate (CYN-PM) and cyanocobalamin-phosphotungstate (CYN-PT) as electroactive materials for sensor I and sensor II. While, sensor III was fabricated using mixed electroactive material (CYN-PM/PT). The fabricated sensors were employed for detection of the investigated drug in its pure form, dosage forms and biological fluids such as human serum and urine. The performance characteristics of the fabricated sensors were investigated and optimized. Under optimum conditions the developed senors were displayed Nernstian slopes of (54.0±0.4, 55.4±0.6 and 57.4±0.8 mV decade-1 at 25°C) at safe pH range 3-8 and concentration ranges of 1.0x10-5-1.0x10-2, 5.0x10-6-1.0x10-2 and 1.0x10-7-1.0x10-2 mol L-1 for sensor I, II and III, respectively. The selectivity coefficientsof the fabricated sensors towards possible interfering species such as cations, amino acids, sugars and some different related pharmacological action drugs. The obtained results were statistically treate4d and compared in terms of student’s t-test and F test with those obtained from other reported methods.

Keywords: Potentiometry, Cyanocobalamin, Coated wire membrane sensor, Pharmaceutical preparations,Biological fluids.

ABSTRACT A field study was carried to evaluate the fate of Acephate + Buprofezin in four different kind of soils i.e. sandy loam, loamy sand, sandy clay and clay soils, following a single application of combinational insecticide formulation of Acephate 50% + Buprofezin 20% WP, supplied by Rallis India Limited, Bangalore, India. The dosage was applied on the moist soil in the plots at @ Acephate + Buprofezin - 1000 g/ha formulation recommended dose and Acephate + Buprofezin - 2000 g/ha formulation doubles the recommended dose. Field experiment plots were laid in Randomized Block Design (RBD) with three replications for each treatment. Untreated control soil plots were maintained for comparison. The composite soil sample was collected from a depth of 0-15 cm 2 hours after the application (0th day), and the sampling was continued at pre-determined intervals (3rd, 5th, 7th, 10th, 20th, 30th and 50th day) after spraying. The collected soil samples were extracted and analyzed for the residues of Acephate by validated Gas chromatograph flame photometry detector (GC-FPD) and Buprofezin High Performance Liquid Chromatography with diode array detector (HPLC-DAD). The terminal residues were re-analyzed by LC-MS/MS in the AJS- ESI source and MRM with positive ion. The half-life for Acephate was DT 50 - 2.6 to 3.1 days and DT 90 - 8.8 to 10.5 days. The half-life for Buprofezin was 11.8 to 19.7 days and DT 90 39.4 to 63.3 days. This followed the first order Kinetics. Keywords: Acephate, Buprofezin, dissipation, HPLC-DAD, GC-FPD, LC-MS/MS.