HPLC method is developed for the estimation of Urea in 2,4-dihydroxy-5-fluoropyrimidine bulk drug and its formulation i.e2,4-dihydroxy-5-fluoropyrimidine Injection by using HPLC system with auto sampler and UV/PDA detector(Column: Atlantis Hilic Silica Column, 250mm x4.6mm, 5 μm, Flow rate: 1.0 mL/min, Column temperature:25°C.Injection Volume: 10 μl, Run time: 25 Minutes Detection wave length: 200 nm),all validation parameters including specificity (interference, forced degradation), Precision (system, method, intermediate ),linearity, accuracy, range, robustness studied. Forced degradation (acid, base,peroxide, water, thermal, humidity, photo stability effect studied for 2,4-dihydroxy-5-fluoropyrimidine in bulk drug & its Injection formulation. Keywords: 2, 4-dihydroxy-5-fluoropyrimidine, Urea limit, Validation, HPLC.

ABSTRACT Alzheimer’s disease will be seen as a great menacing drop inside cognitive and also non-cognitive operate. Typically quick and also long-term memory space will be afflicted although terminology expertise, awareness and also consideration. Because of this the opportunity to understand and also maintain fresh expertise plus the loss in present ones. The present day medicine advancement regarding Alzheimer’s disease started out with all the offer with the cholinergic speculation regarding memory space disadvantages as well as the 1984 study standards regarding Alzheimer’s condition. Simply several cholinesterase inhibitors are usually allowed to industry globally because of the enough basic safety and also efficiency. It is because one other medications analyzed have been unproductive, limited scientific advancement strategies have also been held accountable for your disappointments. The prevention of the particular malfunction regarding acetylcholine simply by inhibition regarding digestive enzymes acetylcholinesterase and also butylcholinesterase together with cholinesterase inhibitors increases the particular cholinergic exhaustion. The latest review article described briefly about the cholinesterase inhibitors namely Donepezil, Rivastigmine, Galantamicyne and Tacrine for the treatment of Alzheimer’s disorder and their clinical pharmacology. Keywords: Alzheimer, Acetylcholinesterase, Donepezil.

ABSTRACT Application of Posidonia Oceanica for the removal of a Cationic dye, Rhodamine B, from aqueous solutions has been investigated. The experiments were carried out in batch mode. Effect of the parameters such as pH, initial dye concentration and temperature on the removal of the dye was studied. Equilibrium was achieved in 60 min. Maximum adsorption of dye was achieved at pH 6.2. Removal percentage was found to be dependent on the initial concentration of dye solution and maximum removal was found to be 88% at 25 mg/L of Rhodamine B. The removal increases from 75% to 88% when the initial concentration of dye solution decreases from 125 mg/L to 50 mg/L. The equilibrium adsorption data were analyzed by Langmuir, Freundlich, Temkin and Dubinin–Radushkevich isotherm models. The (Langmuir) adsorption capacity of the adsorbent is found to be 280 mg/g at 303 K. Kinetic modeling of the process of removal was carried out and the process of removal was found to follow a pseudo second order model. The thermodynamic parameters such as change in free energy (ΔG), enthalpy (ΔH) and entropy (ΔS) were determined and the negative values of ΔG indicated that the process of removal was spontaneous at all values of temperatures. Further, the values of ΔH indicated the endothermic nature of the process of removal. Keywords: Activated Posidonia Oceanica Nano Carbon (PONC); Rhodamine-B dye; Adsorption isotherm; Kinetics; Equilibrium models.

2-aminothiophene forms a significant class of drugs which exhibits the excellent biological activities like anti-inflammatory, antifungal, analgesic, antibacterial, anticancer and antiviral activities. In continuation of our previous vast research on 2-Aminothiophene,the present study is aimed on synthesis of 2-amino 4,5,6,7 tetra hydro benzo[b] thiophene-3-carboxylic ethyl ester derivatives and are characterized by using spectral studies. These derivatives were also screened for their anti-inflammatory activity using carageenan induced rat paw edema using indomethacin as standard. In present research work, the derivatives which are having pyridine substitution [2e] and hydroxyl group [2c] shown significant anti-inflammatory activity. The derivatives [2a, 2b, 2d] containing thiophene were shown moderate activities. Keywords: 2-amino thiophenes, Gewald Synthesis, Anti-inflammatory activity, Carrageenan.

By the reaction of the acid chloride (2) with a various hydrazides (3-6), 1,2,4-triazole-3-thione derivative (7) and piperazine derivatives (8, 9) a series of novel corresponding N’-substituted-(2,4-dioxothiazolidin-5-yl)acetohydrazides (10-13), 5-[2-oxo-2-(4-phenyl-5-thioxo-4,5-dihydro-1H-1,2,4-triazol-1-yl)ethyl]thiazolidine-2,4-dione (14) or 5-[2-oxo-2-(4-substitutedpiperazin-1-yl)ethyl]thiazolidine-2,4-dione (15, 16) derivatives were obtained. All the structures were confirmed by their spectral (1H NMR and 13C NMR) and elemental analysis data. The new compounds were tested for their in vitro antimicrobial activity. Compounds 11-14 had in vitro activity against Gram-positive reference strains of bacteria with MIC values from 62.5 to 250 μg/ml. Keywords: 2,4-thiazolidinedione derivatives, 1,2,4-triazoles, Piperazine derivatives, Antimicrobial activity.