ABSTRACT The present study was undertaken to screen the phytochemicals present in the chloroform extract of Oxystelma esculentum R.Br. The chloroform extract was screened using the standard procedure for UV-Vis spectroscopic, HPLC and FTIR. The UV-Visible spectrum showed the compounds separated at the nm of 400, 450, 500, 536, 606, 664, 700, 750, 800 and 882 with the absorption 3.675, 2.237, 1.734, 1.606, 1.473, 1.931, 1.190, 1.125, 1.071 and 1.010 respectively. The qualitative HPLC fingerprint profile showed ten compounds at different retention times. The profile displayed six prominent peaks at the retention times of 1.650min, 2.113min, 2.347min, 2.433min, 2.800min and 3.013min followed by four moderate peaks were noticed at the retention time of 5.217min, 5.957min, 7.190min and 8.640min. The profile displayed three prominent peaks at the retention times of 1.687min, 2.063min and 2.797min, followed by only one moderate peak at the retention time of 3.000min. The result of FTIR analysis showed the presence of functional groups such as di-sulphate, sulphades, phenyl nucleus, alcohols, hydroxyl group, nitrates, isopropyl, aromatic ring, α-helo and alkanes. Keywords: Oxystelma esculentum, Phytochemicals, Chloroform extract, UV-Visible, HPLC, FTIR.

Two sensitive, precise, accurate and simple methods has been developed and validated for the analysis of Atenolol, in combination with Hydrochlorothiazide and Losartan potassium in tablet dosage form. Method A involved chromatographic estimation of Atenolol in combination with Hydrochlorothiazide and Losartan potassium in tablet dosage form by RP-HPLC. Chromatographic resolution was achieved on a reverse-phase Zorbax SB-C18 (150 x 4.6 mm), 3.5μm column using acetonitrile: water: 0.05mM sodium dihydrogenorthophosphate (pH 2.5) in the ratio of (40:10:50) as mobile phase with a flow rate of 0.7mL/min and isocratic elution with a total run time of 8 minutes. The retention time of Atenolol, Hydrochlorothiazide, Losartan potassium was found to be 1.921, 2.630 and 5.061 respectively. Detection of the multi compounds was carried out at 210nm. The peaks of atenolol, hydrochlorothiazide and losartan potassium were well separated . The Calibration curves were linear over studies ranges with correlation co-efficient found between the range of 0.99 to 1.00. The proposed method is accurate with 99.8% recovery for atenolol, 100.9% recovery for hydrochlorothiazide and 99.6% recovery for losartan potassium and precise (% RSD < 0.5). Keywords: Atenolol, Hydrochlorothiazide, Losartan potassium, HPLC.

Currently  used antimicrobial agents are not very useful due to the resistance developed by the  microbes against them.The present study was aimed at synthesizing Schiff”s base of sulphona mide nucleus incorporated with para-substituted benzaldehyde showing good activity, with para- sulphonamido group playing a key role, and evaluating the potential of this agent as antimicrobial.The improvement achieved in potency of sulfonamide by introducing electron-withdrawing groups at the N1-position, which produced such highly potent drug as sulfadiazine, established the power of molecular modification in drug  discovery.  For  the  accomplishment  of  the  proposed  objective,  the  established  method  of  synthesis  with  some modification was adopted, i.e. refluxing sulphonamide and para substituted derivative of benzaldehyde  in  absolute ethanol for 12-14 hours in water bath in Dean Stark Apparatus.The synthesized compound was subjected to physicochemical and spectral characterization

An isocratic Simultaneous estimation by RP-HPLC Method were developed and validated for the quantification of Dasabuvir, Ombitasvir, Paritaprevir and Ritonavir in tablet dosage form. Quantification was achieved by using a reversedphase C18 column (INERTSIL Column, 5µ, 150 mm ×  4.6 mm) at ambient temper ature  with  mobile phase consisting ofMixed  Ammonium  acetate  Buffer:  Acetonitrile  (40:  60).  The  flow  rate  was  1.0  ml/min.  Measurements  were  made  at  a wavelength of 262nm. The average retention time for Dasabuvir, Ombitasvir, Paritaprevir and Ritonavir were  found to be 2.13 min, 2.82min, 3.90min and 5.75. The proposed method was validated for selectivity, precision, linearity and accuracy. The developed method was successfully applied to estimate the amount of Dasabuvir, Ombitasvir, Paritaprevir and Ritonavir in tablet dosage form.

This paper presents the removal of cationic dye Rhodamine-B from aqueous solutions by using a low cost natural
adsorbent Solanum trilobatum. The effects of various experimental parameters on adsorption such as contact time,
temperature, initial pH, initial dye concentration, adsorbent dosage, ionic strength were examined and the optimal
experimental conditions were evaluated. Adsorption isothermal data could be interpreted by the Langmuir, Freundlich,
Temkin, Dubinin-Radushkevich, Hurkins-Jura, Halsey, Redlich-Peterson, Jovanovich and BET isotherm models equations.
The values of free energy change (ΔG0), enthalpy change (ΔH0), and entropy change (ΔS0) indicated the process to be
spontaneous. Kinetic data have been studied using Elovich and Pseudo-second order equations for understanding the
reaction mechanism

Heterocyclic compounds are known for their biological activities like cardiovascular, vasodilator, anti-arrhythmic, hemodynamic effects, anti-ulcer activity, Calcium antagonistic, spasmolytic activities and angio-tensive converting enzyme inhibition etc. Benzosuberones are a class of heterocyclic compounds which exhibit antimicrobial activity against a variety of bacteria and fungi. In the present study, 2, 3 – dimethyl - 6, 7, 8, 9 - tetrahydro benzocyclohepten-5-one and its derivatives 6-Arylidene -2,3-dimethyl- 6,7,8,9 -tetrahydro- 5H- benzocyclo –hepten -5-one and 2,3 -dimethyl-6,7,8,9-tetrahydrobenzocyclohepten-5-one thiosemi-carbazone are tested for their efficiency as antibacterial agents. Ten bacterial cultures viz. the Gram positive Staphylococcus aureus and Methicillin Resistant Staphylococcus aureus and the Gram negative Escherichia coli, Klebsiella pneumoniae, Citrobacter divergens, Shigella flexneri, Salmonella paratyphi A, Salmonella paratyphi B, Proteus mirabilis and Pseudomonas aeruginosa, isolated from clinical samples were obtained from SVS Medical College, Mahabubnagar, Telangana State, India. The three compounds exhibited antibacterial activity against all the bacteria at different concentrations tested, with Compound 1 showing greater activity against E. coli, Citrobacter divergens, Shigella flexneri, Salmonella paratyphi A, Salmonella paratyphi B and Pseudomonas aeruginosa compared to other two compounds. Similarly, Compound 2 has shown more activity on Methicillin Resistant Staphylococcus aureus than other two compounds and Compound 3 has shown greater activity on Staphylococcus aureus, Klebsiella pneumoniae and Proteus mirabilis than other two compounds. The activity varied with the type of the bacterium and the compound. The Activity Index and Relative Percentage inhibition of the compounds were also estimated. Keywords: Benzosuberones, antibacterial activity, clinical isolates, activity index, relative percentage inhibition.

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Valsartan and Sacubitril, in its pure form as well as in tablet dosage form. Chromatography was carried out on an Sunfire C18 (4.6×250mm) 5μ column using a mixture of Water and Acetonitrile (60:40% v/v) as the mobile phase at a flow rate of 0.9ml/min, the detection was carried out at 220nm. The retention time of the Sacubitril and Valsartan was 3.0, 3.8±0.02min respectively. The method produce linear responses in the concentration range of 5-25μg/ml of Sacubitril and 75-375μg/ml of Valsartan. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations. Keywords: Sacubitril, Valsartan, RP-HPLC, Validation.