A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Tipiracil and Trifluridine, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Hypersil C18 (4.6 x 250mm, 5μm) column using a mixture of Acetonitrile: Water:Methanol (60:20:20v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 230nm. The retention time of the Tipiracil and Trifluridine was 2.8, 3.8 min respectively. The method produce linear responses in the concentration range of 10-50μg/ml for Tipiracil, and 66.6-330μg/ml of Trifluridine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations. Keywords: Tipiracil, Trifluridine, RP-HPLC, PDA Detection, Method validation.

Objective: The purpose of the study is to evaluate 99mTc-5 FU for the detection of sentinel node by Nuclear Imaging (gamma camera and gamma probe). Material and methods:  We have done a study of 3 patients with carcinoma breast who referred for the sentinel node imaging and gamma probe detection. Nuclear Imaging was done by SPECT system and Gamma probe detection done by gamma probe for the detection of sentinel node. Results: Out of three in two patients we have observed the radiocolloid movement and detection of sentinel node has been detected. Conclusion: We have observed that 99mTc-5-FU radiocolloid is a potential radiopharmaceutical for the detection of sentinel node after having further characterization and other tests.

Keywords: Radiocolloid, 99mTc-Fluorouracil (99mTc-5-FU), Scintigraphy, Gamma probe.

The chemistry of pyrimidines and its derivatives has been studied for over a century due to their diverse biological activities. They possess antibacterial, antiviral, antitumor, antihypertensive and antiinflammatory pharmacological activities. Thienopyrimidines formed by the fusion of thiophene moiety with pyrimidine ring, have been reported to be chemotherapeutically active. In view of various biological activities and its enormous importance of thienopyrimidines, we have made an attempt to synthesize and characterize some new 3-[(2-substituted-6,7,8,9-tetrahydro-5h-cyclohepta[b]thieno [2,3-d]pyrimidin-4-yl)amino]propan-1-ol derivatives and evaluate them for anticancer activity. Ethyl 2-amino-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxylate was treated with acetonitrile in presence of hydrochloric acid gas to give 2-methyl-3,5,6,7,8,9-hexahydro-4H-cyclohepta[b]thieno[2,3-d]pyrimidin-4-one, which was reacted with excess POCl3 and then refluxed with dioxane, Triehtylamine and aminopropanol to give 3-[(2-methyl-6,7,8,9-tetrahydro-5H-cyclohepta[b]thieno[2,3-d]pyrimidin-4-yl)amino]propan-1-ol. All the intermediate and final compounds were purified and their chemical structures have been confirmed by IR, 1H NMR and Mass spectral data. All the newly synthesized compounds were screened for their anticancer activity by MTT assay and analyzed statistically. Compounds showed considerable anticancer activity when compared with cyclophosphamide.

Keywords: Anti cancer activity, Synthesis.