The method for the simultaneous estimation of Glimepiride and Metformine hydrochloride from tablet dosage formhas been progress ,establish on simultaneous equation method at duplet determine wavelength 226nm and 232nmrespectively, and also absorbance ratio method at two selected wavelength 251 nm (Iso-absorptive point) and 226 nm (lamdamax of glimepiride). The linearity, was obtained in the concentration ranges of 5-25 μg/ml and 5-25μg/ml for glimepirideand metformine hydrochloride respectively. These method are simple accurate and result of analysis have been validatedstastically and by recovery studies.

A simple, Accurate, precise method was developed for the simultaneous estimation of the Chloramphenicol and Hydrocortisone in Tablet dosage form. Chromatogram was run through Std BDS C18 250 x 4.6 mm, 5m. Mobile phase containing Buffer 0.1% OPA: Acetonitrile taken in the ratio 50:50 was pamped through column at a flow rate of 1 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 245 nm. Retention time of Chloramphenicol and Hydrocortisone were found to be 2.136 min and 2.871 min. %RSD of the Chloramphenicol and Hydrocortisone were and found to be 0.3 and 0.5respectively. %Recovery was obtained as 99.38% and 99.83% for Chloramphenicol and Hydrocortisone respectively. LOD, LOQ values obtained from regression equations of Chloramphenicol and Hydrocortisone were 0.05, 0.14 and 0.26, 0.79 respectively. Regression equation of Chloramphenicol is y = 13708x + 17978, and y = 16395x + 1048 of Hydrocortisone. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries. Keywords: Chloramphenicol, Hydrocartisone, RP-HPLC.

Six novels Schiff base containing 1,3,4-oxadiazole (SB-1 to SB-6) were synthesized by the simple synthesis of
benzohydrazide through nucleophilic acyl substitution reaction which involves a reaction between benzoic acid and hydrazine
hydrate the compound is treated with POCl3and p-amino benzoic acid with the involvement of microwave irradiation (1-2 min
) at 300 watts to obtain the 1,3,4-oxadiazole compound . An equimolar mixture of 0.01 mole of 1, 3, 4-oxadiazole and
substituted benzaldehyde in the presence of glacial acetic acid resulted into respective Schiff base. The newly synthesized
derivatives were characterized by spectroscopical methods using IR, 1HNMR spectroscopy and Mass spectrometry. All the
synthesized compounds were screened for their antibacterial and antifungal activities. Anti-bacterial and anti-fungal activities
were performed by using Agar well diffusion Results of the activities revealed that some of the derivatives showed potent antibacterial and anti-fungal activities and some other compounds shown mild to moderate activities when compared to the
respective reference standard

This paper aims to explore the utilization of enzyme synthesis for modifying antioxidants. Specifically, enzymatic esterification with conjugated linoleic acid (CLA) is investigated as a model for studying the esterification of ascorbic acid. Five different enzymes and four organic solvents were assessed in this study. Among them, Novozym® 435 emerged as the most effective catalyst for producing ascorbyl-CLA ester. The optimal reaction conditions were determined to be at 55°C, with an ascorbic acid to CLA molar ratio of 5. Ionic liquids were employed to facilitate the esterification of polar substrates and eliminate the need for organic solvents. The use of Novozym® 435 as a biocatalyst in the most favorable reaction environment led to the successful production of ascorbyl-CLA ester. Additionally, preliminary optimizations revealed that the solubility of ascorbic acid could be substantially increased (up to 150 g/l) by supersaturating it in ionic liquids, surpassing the solubility achieved in organic solvents (35 g/l). Moreover, elevating the reaction temperature by 70°C further enhanced ascorbic acid solubility in both systems