The main objective was to develop and validate the UV spectrophotometric method for the estimation of
aminophylline in bulk and its tablet dosage formulations as per ICH guidelines. Distilled water is used as a solvent. the λ max
of the Aminophylline was found to be 272 nm. The linearity in the concentration range 5-30 µg/ml with correlation co
efficient of 0.9996. The accuracy studies of proposed method was performed at three different levels, 20%, 40%, 60% and
recovery was found to be in the range of 100.82%. The limit of detection and limit of quantification were found to be 0.0383
and 0.1160 (µg/ml respectively. The % RSD less than 2 which indicates the accuracy and precise of the method the above
method was a rapid tool for routine analysis of aminophylline in the bulk and its tablet dosage forms.

Some novel 4-thiazolidinone derivatives have been synthesized by the condensation of isatin/5-chloroisatin with
thiosemicarbazide to yield thiosemicarbazones, which were then cyclized to form corresponding thia-3,4, 9-triaza-fluoren-2-
ylamines. These were reacted with substituted aldehydes to give corresponding Schiff bases, which were cyclized using
thioglycolic acid in the presence of zinc chloride to obtain the 4-thiazolidinone derivatives. All the synthesized compounds
were characterized by spectral (IR, MS and NMR) and elemental analysis. The compounds were screened for their
antibacterial activity against Gram-positive bacteria (B. subtilis, S. aureus, B. pumilus and M. luteus), Gram-negative bacteria
(P. aeruginosa, E. coli and P. fluorescens) and for antifungal activity against A. niger and P. chrysogenum by agar-diffusion
method. The minimum inhibitory concentrations of these compounds were also determined by tube dilution method. The
antimicrobial effectiveness of all the compounds was found to be concentration dependent. Two compounds—2- methyl-3-
(1-thia-3, 4, 9-triaza-fluoren-2-yl)-thiazolidin-4- one (7aI) and 2-naphthalen-1-yl-3-(1-thia-3, 4, 9-tri aza-fluoren-2-yl)-
thiazolidin-4-one (7aII)—exhibited good antibacterial activity. The antibacterial activity of all the compounds was found to
be better than the antifungal activity.

To develop a simple, cheap, accurate, and rapid Reverse Phase High Performance Liquid Chromatographic (RPHPLC) method and validate as per ICH guidelines for estimation of donepezil and memantine in pharmaceutical dosage
forms. The separation was conducted by using mobile phase consisting of phosphate buffer: methanol in the ratio (70:30).
The wavelength was found at 273nm. HPLC alliance with empower software is used for chromatographic determination. The
separation was conducted by using inertsil C-18 (4.6×250mm×5μm) at the flow rate of 1.0 ml/min using variable wavelength
detector. The developed method resulted in donepezil and memantine eluting at 5.067 min and 2.003min. The method was
found to be linear over the concentration range 25-125μg/ml with coefficient regression R2-0.999. Mean recovery was found
to be in the range of 99.8%, during accuracy studies. The limit of detection (LOD) was found to be 2.75mg/ml and
3.14mg/ml. The limit of quantitation (LOQ) was found to be 9.96mg/ml and 10.05 mg/ml respectively. A cheap, accurate,
precise, linear and rapid RP-HPLC method was developed and validated for the quantitative estimation of Donepezil and
memantine as per ICH guidelines.

Tuberculosis remains a worldwide public health problem despite the fact that the causative organisms was discovered more than 100 years ago and highly effective drugs and vaccines are available making tuberculosis a preventable and curable disease. In this paper we report the molecular docking studies of 30 quinazoline derivatives having antitubercular activity. The derived compounds were analyzed for drug likeliness based on the Lipinski’s rule of Five and docking study was performed between receptor and ligands by Autodock vina with PyRx and visualized by Biovia Discovery studio 2020 client. Docking studies have shown that the quinazoline derivatives interacts and bind efficiently with 1P44 (enoyl-acyl carrier protein reductase (InhA)) enzyme which resulted in antitubercular activity.

Lupeol is a triterpenoid pentacyclic compound that is known for its anti-inflammatory and anti-cancer properties. Its
activities are based on its anti-oxidant potential. Lupeol has been found to have a contraceptive effect due to its inhibiting
effect on the calcium channel of sperm (CatSper). Lupeol has also been shown to exert anti-angiogenic and anti-cancer
effects via the downregulation of TNF-alpha and VEGFR-2. Famous anti-inflammatory ethno-medicinal plant Camellia
japonica contains anti-inflammatory component lupeol in its leaf. In order to determine the drug in biological fluid or in
pharmaceutical preparations, there are no. of methods available, that is HPTLC, HPLC, and spectrophotometery. It can be
concluded that the proposed method is simple, rapid, accurate, precise, economic and reproducible for UV spectrophotometric estimation of lupeol from pharmaceutical formulation. This method can be successfully applied for routine
estimation of lupeol in bulk and pharmaceutical dosage form.